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pcrA [2019-06-24 09:10:12]
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pcrA [2019-06-24 09:10:12]

ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
locus
BSU06610
pI
5.77
mw
83.37 kDa
protein length
739 aa Sequence Blast
gene length
2220 bp Sequence Blast
function
plasmid rolling-circle replication, conjugative transfer of ICEBs1
product
ATP-dependent DNA helicase
essential
yes
synonyms
yerF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.2|Mobile genetic elements] → [category|SW 5.2.2|Additional genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    719,370 → 721,589

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]
  • dismantles [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] filaments [Pubmed|20723756]

    • Protein family

  • [SW|helicase family] (according to UniProt)
  • UvrD subfamily (with [protein|4DE90422754DA1FD9EFE58766EACB99CE6171207|HelD] and [protein|B0789A727D9E3578FF1CB6162F365F45FADB0B3E|YjcD]) (according to UniProt)

    • Paralogous protein(s)

  • [protein|B0789A727D9E3578FF1CB6162F365F45FADB0B3E|YjcD]

    • [SW|Domains]

  • two RecA-like domains [Pubmed|24147116]
  • intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]

    • Effectors of protein activity

  • unwinding of the double-stranded ICEBs1 DNA is stimulated by [protein|search|HelP] [Pubmed|23326247]
  • helicase activity is stimulated by interaction with [SW|RNA polymerase] [Pubmed|24147116]

    • Structure

  • [PDB|2PJR] (product complex, complex with sulphate ion, ''Geobacillus stearothermophilus'')
  • [PDB|1PJR] (substrate complex, complex with DNA, ''Geobacillus stearothermophilus'')
  • [PDB|5DMA], C-terminal [SW|RNA polymerase] interaction domain [pubmed|28160601]

    • Expression and Regulation

    Operons

    genes
    [gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]-[gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA]-[gene|03EFF93C1B0CE075AE068ECC4EDE9BE5D03C857E|ligA]-[gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]
    description
    [Pubmed|9701819]

    regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

    • regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]
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    Biological materials

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

    • References

    Reviews

  • 19943906,29856930

    • Original Publications

  • 12073041,9701819,16267290,19943900,20723756,18276648,17449621,10899133,10199404,23326247,24147116,21710567,26070154,28160601